Description

• Title:
  Regulation of the abundance of Y‑family polymerases in the cell cycle of budding yeast in response to DNA damage
• Creator:
  Sobolewska, Aleksandra;
  Halas, Agnieszka;
  Plachta, Michal;
  McIntyre, Justyna;
  Sledziewska-Gojska, Ewa
• Description:
  Y-family DNA polymerases mediate DNA damage tolerance via translesion synthesis (TLS). Because of the intrinsically error-prone nature of these enzymes, their activities are regulated at several levels. Here, we demonstrate the common regulation of the cellular abundance of Y-family polymerases, polymerase eta (Pol eta), and Rev1, in response to DNA damage at various stages of the cell cycle. UV radiation influenced polymerase abundance more when cells were exposed in S-phase than in G1- or G2-phases. We noticed two opposing effects of UV radiation in S-phase. On one hand, exposure to increasing doses of UV radiation at the beginning of this phase increasingly delayed S-phase progression. As a result, the accumulation of Pol eta and Rev1, which in nonirradiated yeast is initiated at the S/G2-phase boundary, was gradually shifted into the prolonged S-phase. On the other hand, the extent of polymerase accumulation was inversely proportional to the dose of irradiation, such that the accumulation was significantly lower after exposure to 80 J/m2 in S-phase than after exposure to 50 J/m2 or 10 J/m2. The limitation of polymerase accumulation in S-phase-arrested cells in response to high UV dose was suppressed upon RAD9 (but not MRC1) deletion. Additionally, hydroxyurea, which activates mainly the Mrc1-dependent checkpoint, did not limit Pol eta or Rev1 accumulation in S-phase-arrested cells. The results show that the accumulation of Y-family TLS polymerases is limited in S-phase-arrested cells due to high levels of DNA damage and suggest a role of the Rad9 checkpoint protein in this process.
• Object availability:
  free access
• Date:
  2020
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Relation: http://eprints.ibb.waw.pl/1902 ;https://link.springer.com/article/10.1007/s00294-020-01061-3
• Subject:
  Q Science (General)
• Identifier: http://eprints.ibb.waw.pl/1902/2/Sobolewska2020_RegulationOfTheAbundanceOfY-family.pdf ;
  Sobolewska, Aleksandra and Halas, Agnieszka and Plachta, Michal and McIntyre, Justyna and Sledziewska-Gojska, Ewa (2020) Regulation of the abundance of Y‑family polymerases in the cell cycle of budding yeast in response to DNA damage. Current Genetics, 66 (4). pp. 749-763. ISSN 0172-8083
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other