Description

• Title:
  SUPEROVULATION NEW MODELS INCLUDING FSH DOSE REDUCTION AND NEW PREPARATIONS
• Creator:
  Jaskowski, Jędrzej
• Description:
  It was estimated that every year around the world, approximately half a million bovineembryos are produced (Cremonesi, 2020). The classic superovulation program involvesadministering of a series of FSH injections to embryo donor cows starting on day 9-12 of theoestrus cycle. In Europe, Pluset and Folltropin preparations are commercially used for thispurpose, both available on the market in Poland (Kulus, 2019). This is the mechanismunderlying superovulatory protocols that were developed in the 1980s, which were furtherimproved by refining hormonal preparations like human menopausal gonadotropin (HMG),equine chorionic gonadotropin (eCG), or follicle-stimulating hormone (FSH) from porcine orovine pituitaries (Cremonesi 2020). Superovulation protocols vary widely based on the FSHsource, the diluent used, the number and timing of FSH injections and the timing andutilisation of various prostaglandins, controlled internal progesterone releasing devices,gonadotrophin-releasing hormone, and other means of controlling follicular development andovulation (Mikkola, 2019). These may include nutritional status, reproductive history, age,season, breed, effects of repeated superovulations and ovarian status at the time oftreatment (Cremonesi 2020). An approach that has shown promise is to initiate FSHtreatments at the time of the emergence of the first follicular wave following GnRH-inducedovulation. Using of aspiration of dominant follicle is possible to induce new follicular wave.This simple biotechnical treatment has a final impact on the number of in vivo producedembryos. Its seems, that of the interval from follicle aspiration to initiation of lengthened FSHtreatment (Cirit, 2019). However, it has been shown that it may be possible to ignorefollicular wave status, and by extending the treatment protocol induce smaller follicles togrow and reach maturity and superovulate. Finally, the short halflife of pituitary FSHnecessitates twice daily treatments which are time-consuming, stressful and subject to error.Recent treatment protocols have permitted superstimulation with a single or alternatively,two FSH treatments, reducing the need for animal handling during FSH treatment (Mapletoft,2013). A single dose protocol of FSH for superstimulation in cattle may improve complianceand superovulatory response. A single subcutaneous (sc) administration of pFSH wasefficacious, but response depended on body condition and injection site; the adipose tissuepad behind the shoulder was most efficacious. Inconsistent results in Holsteins were partiallyovercome by sc administration of 75% of the total pFSH dose behind the shoulder on the firstday followed by 25% 48 h later (Bo, 2018). The split-single injection given ischiorectal fossa(split-single IRF administration had a comparable superovulatory response to the traditionaltwice-daily protocol. Moreover, the ovulation rate, ovarian follicle responses, and embryoquality were affected by heat stress (Chumchai, 2021, Ratsiri, 2022). In the other study theovarian responses in the split-single IRF group were similar to those of the control group (p >.05) but higher compared with the split-single IM group. Regardless of the route ofadministration of FSH. The high THI affected ovulation rate as well as the numbers oftransferable embryos and degenerated embryos (Thanaporn, 2021). A recombinant longactingovine follicle stimulating hormone (roFSH) has been devised and its biologicaleffectiveness following a single dose has been assessed in several experiments under fieldconditions, in pasture-based beef and dairy farming in New Zealand. Owing to the molecularstructure of this long-acting roFSH, which includes additional N-glycosylation sites, a singledose combined with a simple CIDR-based superovulatory regime elicits successful ovarianstimulation with averages of 11.8 corpora lutea and of 6.1 good quality embryos collected incattle. Solid performance of this novel FSH was demonstrated in several beef and dairybreeds which included yearling heifers and mixed age cows, with embryo production resultsin the same range as those observed nowadays with eight doses of commercial pituitary FSH(pFSH). Viable embryos produced from these collections, when implanted either fresh orfrozen and thawed, gave pregnancy rates in recipients similar to those collected from cowsand heifers superstimulated with pFSH. Repeated superovulatory treatment of the same cowswas not associated with a decrease in ovarian response or embryo yield. The singleadministration of this long-acting roFSH when combined with a modified simplesuperovulatory regime has the advantage of reducing animal welfare concerns, loweringlabour resource requirements and giving similar results to other commercially preparedpituitary FSH extracts (Sanderson, 2020, Gutierrez-Rreinoso, 2022). It is well known that 70%of embryos are produced by 30% of donors. This very high variability was tried to bereduced, with very good results by administrating platelet rich plasma (PRP) inside the ovarybefore the superovulation protocol. This hemocomponent is rich in growth factors andcytokines known for their regenerative properties in human and veterinary medicine(Cremonesi, 2020, Cirit, 2020).
• Object availability:
  free access
• Rights: https://creativecommons.org/licenses/by-nd/4.0 ;
  Prawa autorskie (c) 2023 Jaśkowski J.M.
• Date:
  2023.07.09
• Type:
  info:eu-repo/semantics/article;
  info:eu-repo/semantics/publishedVersion
• Language:
  English
• Source:
  Translational Research in Veterinary Science; Vol. 6 No. 1 (2023); 9-10;
  Translational Research In Veterinary Science; Tom 6 Nr 1 (2023); 9-10;
  2657-4489
• Format:
  application/pdf
• Publisher:
  Nicolaus Copernicus University in Toruń
• Relation: https://apcz.umk.pl/TRVS/article/view/63486/43170
• Subject:
  superovulation;
  results improvement;
  cows
• Identifier: https://apcz.umk.pl/TRVS/article/view/63486
• Data provider:
  Akademicka Platforma Czasopism
• Can I use it?:
  seek permission
• Type:
  text