Object's details: Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging
Creator:
Enkhbaatar, Tuguldur;
Skoneczny, Marek;
Stępień, Karolina;
Mołoń, Mateusz;
Skoneczna, Adrianna
Description:
Aging is inevitable and affects all cell types, thus yeast cells are often used as a model in aging studies. There are two approaches to studying aging in yeast: replicative aging, which describes the proliferative potential of cells, and chronological aging, which is used for studying post-mitotic cells. While analyzing the chronological lifespan (CLS) of diploid Saccharomyces cerevisiae cells, we discovered a remarkable phenomenon: ploidy reduction during aging progression. To uncover the mechanism behind this unusual process we used yeast strains undergoing a CLS assay, looking for various aging parameters. Cell mortality, regrowth ability, autophagy induction and cellular DNA content measurements indicated that during the CLS assay, dying cells lost their DNA, and only diploids survived. We demonstrated that autophagy was responsible for the gradual loss of DNA. The nucleophagy marker activation at the start of the CLS experiment correlated with the significant drop in cell viability. The activation of piecemeal microautophagy of nucleus (PMN) markers appeared to accompany the chronological aging process until the end. Our findings emphasize the significance of maintaining at least one intact copy of the genome for the survival of post-mitotic diploid cells. During chronological aging, cellular components, including DNA, are exposed to increasing stress, leading to DNA damage and fragmentation in aging cells. We propose that PMN-dependent clearance of damaged DNA from the nucleus helps prevent genome rearrangements. However, as long as one copy of the genome can be rebuilt, cells can still survive.
Object availability:
free access
Rights:
cc_by
Date:
2023.10.09
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
IMPACT JOURNALS LLC
Relation: http://eprints.ibb.waw.pl/2334 ;https://www.aging-us.com/article/205102/text ;doi: 10.18632/aging.205102.
Subject:
QD Chemistry;
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/2334/1/aging-v15i19-205102.pdf ;
Enkhbaatar, Tuguldur and Skoneczny, Marek and Stępień, Karolina and Mołoń, Mateusz and Skoneczna, Adrianna (2023) Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging. Aging (Albany NY), 15 (19). pp. 9965-9983. ISSN 1945-4589
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging

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