Object's details: An integrated LC-ESI-MS platform for quantitation of serum peptide ladders. Application for colon carcinoma study.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
An integrated LC-ESI-MS platform for quantitation of serum peptide ladders. Application for colon carcinoma study.
Creator:
Bakun, Magda;
Karczmarski, Jakub;
Poznański, Jarosław;
Rubel, Tymon;
Rózga, Małgorzata;
Malinowska, Agata;
Sands, Dorota;
Hennig, Ewa E.;
Oledzki, Janusz;
Ostrowski, Jerzy;
Dadlez, Michal
Description:
Mounting evidence indicates that MS analysis of the human blood peptidome allows to distinguish between cancer and non-cancer samples, giving promise for a new MS-based diagnostic tool. However, several aspects of already published work have been criticized and demand for more methodical approach has been formulated. Motivated by this we undertook a systematic study of the plasma and serum peptidome using an integrated ESI-LC-MS-based platform, equipped with new data analysis tools for relative and absolute peptide quantitation. We used a high resolution LC-ESI-MS to analyze well-separated MS signals corresponding to peptides, and measured the variability of >1000 peptide signal amplitudes across a set of plasma and serum samples from healthy individuals. By spiking serum samples with known amounts of isotopically labeled versions of a selected set of peptides we measured the variability of their absolute concentration in this sample set and demonstrated a strong influence of clotting time on the concentration of these peptides in serum. Finally, we used this new LC-ESI-MS analytical platform for the differential analysis of healthy versus colon cancer serum samples and found that it was possible to distinguish the two groups with 89.8% sensitivity and 94.6% specificity.
Object availability:
free access
Date:
2009.08
Type:
Article;
PeerReviewed
Relation: http://eprints.ibb.waw.pl/304
Subject:
R Medicine (General);
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Identifier:
Bakun, Magda and Karczmarski, Jakub and Poznański, Jarosław and Rubel, Tymon and Rózga, Małgorzata and Malinowska, Agata and Sands, Dorota and Hennig, Ewa E. and Oledzki, Janusz and Ostrowski, Jerzy and Dadlez, Michal (2009) An integrated LC-ESI-MS platform for quantitation of serum peptide ladders. Application for colon carcinoma study. Proteomics. Clinical applications, 3 (8). pp. 932-46. ISSN 1862-8354
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: An integrated LC-ESI-MS platform for quantitation of serum peptide ladders. Application for colon carcinoma study.

Description

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