Description

• Title:
  NUMBER OF TOTAL OVA AND EMBRYOS FROM FLUSHED DONOR COWS DEPENDING ON THE SKILLS OF TECHNICAL PERSONNEL
• Creator:
  Grzeczka, Arkadiusz;
  Sobolewski, Jarosław
• Description:
  One of the essential conditions for the successful acquisition of embryos is their perfectsearch. Experience of the person or team searching for the embryos seems to be of keyimportance in these cases. In commercial activities, we usually worked with one or twopeople with different qualifications. Their results in terms of the number of recoveredembryos and oocytes (TOE) compared to ultrasound-detected corpora lutea were different.We therefore assumed that the success of an embryo search is critically dependent on theskill and experience of the person or embryo search team. The evaluation is based on resultsof embryo collection from 153 heifers and embryo donor cows. The donor was subjected to astandard procedure of superovulation with the use of FSH (Pluset Calier). On the 7th day afteroestrus, the number of corpora lutea was counted in all females based on the ultrasoundexamination (iSkan Draminski), then the females were washed out. For this purpose, aNeustadt Aisch catheter was used (Minitübe). After sedimentation of the obtained fluid,embryosearch was performed under a stereoscopic microscope and magnification of 70X.Search was conducted by 11 teams – consisting of one or two people, differing in seniorityand experience by comparing the values of Paerson correlation coefficients. Correlationcoefficients R at P<0.05 were considered highly correlated, R values at P>0.05-0.065 wereconsidered as showing a correlation trend, R values at P>0.065 were considereduncorrelated. The number of corpora lutea (NCL) averaged 9.83 whereas the number ofembryos and oocytes – 6.03 (TRE). There was a clear relationship between the number ofembryos and oocytes retrieved and the individuals or team searching for the embryos. Thissupports the thesis that the selection and training of embryo search technicians is critical tothe success of the ET team. On the other hand, the discrepancies between the number of CLand TRE found in the ovaries could be due to the influence of other factors during thewashout of the embryos.
• Object availability:
  free access
• Rights: https://creativecommons.org/licenses/by-nd/4.0 ;
  Prawa autorskie (c) 2023 Grzeczka A, Jaśkowski B.M.
• Date:
  2023.07.09
• Type:
  info:eu-repo/semantics/article;
  info:eu-repo/semantics/publishedVersion
• Language:
  English
• Source:
  Translational Research in Veterinary Science; Vol. 6 No. 1 (2023); 8;
  Translational Research In Veterinary Science; Tom 6 Nr 1 (2023); 8;
  2657-4489
• Format:
  application/pdf
• Publisher:
  Nicolaus Copernicus University in Toruń
• Relation: https://apcz.umk.pl/TRVS/article/view/63485/43169
• Subject:
  number of embryos;
  technical personnel;
  skills
• Identifier: https://apcz.umk.pl/TRVS/article/view/63485
• Data provider:
  Akademicka Platforma Czasopism
• Can I use it?:
  seek permission
• Type:
  text