Description

• Title:
  The Oxidative Stress May be Induced by the Elevated Homocysteine in Schizophrenic Patients
• Creator:
  Dietrich-Muszalska, Anna;
  Malinowska, Joanna;
  Olas, Beata;
  Głowacki, Rafał;
  Bald, Edward;
  Wachowicz, Barbara;
  Rabe-Jabłońska, Jolanta
• Contributor:
  A. Dietrich-Muszalska J. Rabe-Jabłońska Department of Affective and Psychotic Disorders, Medical University of Lodz, Czechoslowacka 8/10, 92-216 Lodz;
  J. Malinowska B. Olas (&) B. Wachowicz Department of General Biochemistry, University of Lodz, Pomorska 141/3, 90-236 Lodz;
  R. Głowacki E. Bald Department of Environmental Chemistry, University of Lodz, Pomorska 163, 90-236 Lodz
• Description:
  The mechanisms of oxidative stress in schizophrenic patients are not fully understood. In the present study, we investigated the effect of elevated level of homocysteine (Hcys) on some parameters of oxidative stress, namely thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation in plasma, the level of carbonyl groups in plasma proteins, as well as the amount of 3-nitrotyrosine in plasma proteins isolated from schizophrenic patients. Patients hospitalised in I and II Psychiatric Department of Medical University in Lodz, Poland were interviewed with special questionnaire (treatment, course of diseases, dyskinesis and other EPS). According to DSM-IV criteria all patients had diagnosis of paranoid type. They were treated with antipsychotic drugs (clozapine, risperidone, olanzapine). Mean time of schizophrenia duration was about 5 years. High-performance liquid chromatography was used to analyse the total level of homocysteine in plasma. Levels of carbonyl groups and 3-nitrotyrosine residues in plasma proteins were measured by ELISA and a competition ELISA, respectively. The lipid peroxidation in plasma was measured by the level of TBARS. Our results showed that in schizophrenic patients the amount of homocysteine in plasma was higher in comparison with the control group. We also observed a statistically increased level of biomarkers of oxidative/nitrative stress such as carbonyl groups or 3-nitrotyrosine in plasma proteins from schizophrenic patients. Moreover, our experiments indicate that the correlation between the increased amount of homocysteine and the oxidative stress exists. Considering the data presented in this study, we suggest that the elevated Hcys in schizophrenic patients may stimulate the oxidative stress.
• Object availability:
  free access
• Date:
  2015.02.13;
  2012
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Springer
• Relation:
  Neurochemical Research;2012/37
• Subject:
  Schizophrenic disorders;
  Oxidative stress;
  Carbonyl group;
  Homocysteine
• Identifier:
  1573-6903;
  http://hdl.handle.net/11089/6695 ;
  10.1007/s11064-012-0707-3
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  seek permission
• Type:
  image