Description

• Title:
  Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
• Creator:
  Kubalczyk, Paweł;
  Purgat, Krystian;
  Głowacki, Rafał;
  Kośka, Izabella
• Contributor:
  Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Lodz, Poland;
  Doctoral School of Exact and Natural Sciences, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland;
  pawel.kubalczyk@chemia.uni.lodz.pl;
  krystian.purgat@edu.uni.lodz.pl;
  rafal.glowacki@chemia.uni.lodz.pl;
  izabella.koska@edu.uni.lodz.pl
• Description:
  We have developed a precise and accurate method for the determination of ciprofloxacin and ofloxacin in meat tissues. Our method utilizes capillary electrophoresis with a transient pseudoisotachophoresis mechanism and liquid–liquid extraction during sample preparation. For our experiment, a meat tissue sample was homogenized in pH 7.00 phosphate buffer at a ratio of 1:10 (tissue mass: buffer volume; g/mL). The extraction of each sample was carried out twice for 15 min with 600 µL of a mixture of dichloromethane and acetonitrile at a 2:1 volume ratio. We then conducted the electrophoretic separation at a voltage of 16 kV and a temperature of 25 ◦C using a background electrolyte of 0.1 mol/L phosphate–borate (pH 8.40). We used the UV detection at 288 nm. The experimentally determined LOQs for ciprofloxacin and ofloxacin were 0.27 ppm (0.8 nmol/g tissue) and 0.11 ppm (0.3 nmol/g tissue), respectively. The calibration curves exhibited linearity over the tested concentration range of 2 to 10 nmol/g tissue for both analytes. The relative standard deviation of the determination did not exceed 15%, and the recovery was in the range of 85–115%. We used the method to analyze various meat tissues for their ciprofloxacin and ofloxacin contents.
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 4.0 Międzynarodowe;
  http://creativecommons.org/licenses/by/4.0
• Date:
  2021.12.16;
  2021
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  MDPI
• Relation:
  Molecules;26(22);
  6931
• Subject:
  capillary electrophoresis;
  transient pseudo-isotachophoresis;
  extraction;
  antibiotics;
  ciprofloxacin;
  ofloxacin
• Identifier:
  Kośka, I.; Purgat, K.; Głowacki, R.; Kubalczyk, P. Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis. Molecules 2021, 26, 6931. https:// doi.org/10.3390/molecules26226931;
  http://hdl.handle.net/11089/40066 ;
  10.3390/molecules26226931
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image