Description

• Title:
  Genetic engineering of low‑temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow
• Creator:
  Grzesiak, Jakub;
  Rogala, Małgorzata Marta;
  Gawor, Jan;
  Kouřilová, Xenie;
  Obruča, Stanislav
• Description:
  In recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 °C when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17.5 °C. The PHB yield did not increase considerably or even decreased when carbon source concentration exceeded 10 g/L hinting that the strain is oligotrophic in nature. The strain was also capable of introducing 3-hydroxyvalerate (3HV) into the polymer structure, which is known to improve PHA thermoplastic properties. This is the first investigation providing insight into a PHA biosynthesis process by means of a true psychrophile, offering guidelines on polar-region bacteria cultivation, production of PHA and also on the methodology for genetic engineering of psychrophiles.
• Object availability:
  free access
• Date:
  2023
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Springer Japan
• Relation: http://eprints.ibb.waw.pl/2375 ;https://link.springer.com/article/10.1007/s00792-023-01311-5
• Subject:
  QR Microbiology
• Identifier: http://eprints.ibb.waw.pl/2375/1/s00792-023-01311-5.pdf ;
  Grzesiak, Jakub and Rogala, Małgorzata Marta and Gawor, Jan and Kouřilová, Xenie and Obruča, Stanislav (2023) Genetic engineering of low‑temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow. Extremophiles, 27 . ISSN 1431-0651
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other